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Volcano Plot Visualization

volcano_plot.Rd

Uses ggplot2, ggrepel, and tidyverse to construct a volcano plot from differential expression results.

Usage

volcano_plot(
  diffexp_df,
  log2fc_thresh = 0,
  p.val_adj_thresh = 0.05,
  lower_xlim = -5,
  upper_xlim = 5,
  step = 1
)

Arguments

diffexp_df

A tibble or data frame containing differential expression results. Must contain, at minimum, p.val_adj and log2FC columns.

log2fc_thresh

Numeric threshold for the absolute log2 fold-change used to identify differentially expressed genes for display.

p.val_adj_thresh

Numeric threshold for the adjusted p-value used to identify statistically significant differentially expressed genes.

lower_xlim

Numeric value specifying the lower limit of the x-axis. Should be less than 0. For example, -5.

upper_xlim

Numeric value specifying the upper limit of the x-axis. Should be greater than 0. For example, 5.

step

Numeric value specifying the interval between x-axis ticks.

Value

A list containing:

  • plot: A ggplot object containing the volcano plot.

  • data: A data frame containing the differential expression results used to construct the plot.